Restriction Fragment Length Polymorphism (RFLP)

Variable Number Tandem Repeats (VNTR’s)

Overview of RFLP Process

DNA Extraction

Restriction Enzymes

Electrophoresis

Southern Blotting

Probe Hybridization

Autoradiography

PCR Process

PCR Round 1

PCR Round 2

Polymarker Dot-blot

Mitochondrial DNA

• Contains only maternal DNA
• Contains one-half of DNA material
• Excellent for tracing lineage through maternal line

The Elisha Wade kidnapping case:

1. Why did suspicion first fall on Elisha's father?
 
 
 
 
 

2. What type of sample was the DNA test ultimately done on?
 
 

3. What kind of DNA typing was done in this case?
 
 

4. Was it considered to be individual evidence?
 
 
 

5. With respect to Elisha's father, what were the results of the DNA analysis?
 
 
 
 

The Colin Pitchfork Case

1. What unusual method did the police use to try to flush out the killer?
 
 
 
 

2. What type of DNA typing was done in this case?
 
 
 

3. What did the results of the cumulative DNA tests show?
 
 
 
 

4. What did Dr. Blake have to say about the use of the term "match" in describing DNA comparisons?
 
 
 
 
 

5.  Why did Dr. Blake say that DNA typing was not like fingerprint analysis?
 

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